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Title: Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
Authors: Bresolin, Iara Rocha Antunes Pereira [UNIFESP]
Bresolin, Igor Tadeu Lazzarotto [UNIFESP]
Silveira, Edgar
Tambourgi, Elias Basile
Mazzola, Priscila Gava
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia Instituto de Genética e Bioquímica
Keywords: Bromelain
pineapple peel
downstream processing
therapeutic applications
enzymatic activity
Issue Date: 1-Dec-2013
Publisher: Instituto de Tecnologia do Paraná - Tecpar
Citation: Brazilian Archives of Biology and Technology. Instituto de Tecnologia do Paraná - Tecpar, v. 56, n. 6, p. 971-979, 2013.
Abstract: The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
ISSN: 1516-8913
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