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Title: Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
Authors: Gelatti, Luciane Cristina
Bonamigo, Renan Rangel
Inoue, Fernanda Matsiko [UNIFESP]
Carmo, Mirian Silva do [UNIFESP]
Becker, Ana Paula
Castrucci, Fernanda Marques da Silvan [UNIFESP]
Pignatari, Antonio Carlos Campos [UNIFESP]
d'Azevedo, Pedro Alves [UNIFESP]
Universidade Federal de Ciências da Saúde de Porto Alegre Laboratório de Cocos Gram-positivos
Faculdade Serra da Mesa
Universidade Federal de São Paulo (UNIFESP)
Keywords: Staphylococcus aureus
Skin infections
Soft tissue infections
Issue Date: 1-Jan-2013
Publisher: Sociedade Brasileira de Medicina Tropical - SBMT
Citation: Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 1, p. 34-38, 2013.
Abstract: INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
ISSN: 0037-8682
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