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dc.contributor.authorMatsumoto, Cristianne Kayoko [UNIFESP]
dc.contributor.authorChimara, Erica
dc.contributor.authorRamos, Jesus Pais
dc.contributor.authorCampos, Carlos Eduardo Dias
dc.contributor.authorCaldas, Paulo Cesar de Souza
dc.contributor.authorLima, Karla Valeria Batista
dc.contributor.authorLopes, Maria Luiza
dc.contributor.authorDuarte, Rafael Silva
dc.contributor.authorLeao, Sylvia Cardoso [UNIFESP]
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 969-977, 2012.
dc.description.abstractA single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.en
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.rightsAcesso aberto
dc.subjectMycobacterium abscessusen
dc.subjectrapid testen
dc.subjectepidemiological surveillanceen
dc.titleRapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazilen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInstituto Adolfo Lutz
dc.contributor.institutionEscola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio Fraga
dc.contributor.institutionInstituto Evandro Chagas
dc.contributor.institutionUniversidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Góes
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e Parasitologia
dc.description.affiliationInstituto Adolfo Lutz
dc.description.affiliationEscola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio Fraga
dc.description.affiliationInstituto Evandro Chagas
dc.description.affiliationUniversidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Góes
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Microbiologia, Imunologia e Parasitologia
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