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Title: Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil
Authors: Matsumoto, Cristianne Kayoko [UNIFESP]
Chimara, Erica
Ramos, Jesus Pais
Campos, Carlos Eduardo Dias
Caldas, Paulo Cesar de Souza
Lima, Karla Valeria Batista
Lopes, Maria Luiza
Duarte, Rafael Silva
Leao, Sylvia Cardoso [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Instituto Adolfo Lutz
Escola Nacional de Saúde Pública-Fiocruz Centro de Referência Professor Hélio Fraga
Instituto Evandro Chagas
Universidade Federal do Rio de Janeiro Instituto de Microbiologia Professor Paulo de Góes
Keywords: Mycobacterium abscessus
rapid test
epidemiological surveillance
Issue Date: 1-Dec-2012
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 969-977, 2012.
Abstract: A single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.
ISSN: 0074-0276
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