Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/6896
Title: Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes
Authors: Rasmussen, Lucas Trevizani [UNIFESP]
Lábio, Roger Willian de
Caleman Neto, Agostinho
Silva, Luiz Carlos da
Queiroz, Valdeir Fagundes de
Smith, Marilia de Arruda Cardoso [UNIFESP]
Payão, Spencer Luiz Marques [UNIFESP]
Sacred Heart University Postgraduate Program in Oral Biology
Marília Medical School Blood Center Department of Genetics
Marília Medical School Department of Anatomic Pathology
Marília Medical School Department of Digestive System Surgery
Universidade Federal de São Paulo (UNIFESP)
Keywords: Helicobacter pylori
cagA
vacA
dental plaque
dyspeptic patient
saliva
Issue Date: 1-Jan-2012
Publisher: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Citation: Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 18, n. 2, p. 180-187, 2012.
Abstract: Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated with those from the oral cavity. There was a high H. pylori frequency in patients while the cagA gene was associated with vacA s1 alleles in gastric biopsies. Our results suggest a reservoir of the species in the oral cavity and that, in one patient, more than one H. pylori strain may exist in the saliva, dental plaque and stomach. We found a relationship between gastric infection and the bacterium in the oral cavity, with the cytotoxin genotype varying between saliva and dental plaque.
URI: http://repositorio.unifesp.br/handle/11600/6896
ISSN: 1678-9199
Other Identifiers: http://dx.doi.org/10.1590/S1678-91992012000200008
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