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Title: New multilocus sequence typing of MRSA in São Paulo, Brazil
Authors: Carmo, Mirian Silva do [UNIFESP]
Inoue, Fernanda [UNIFESP]
Andrade, Soraya Sgambatti [UNIFESP]
Paschoal, Luiz [UNIFESP]
Silva, Fernanda M. [UNIFESP]
Oliveira, Vitor [UNIFESP]
Pignatari, Antonio Carlos Campos [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Centro Universitário Fundação Instituto de Ensino para Osasco
Keywords: Methicillin-resistant Staphylococcus aureus
Molecular sequence typing
Molecular typing
Issue Date: 1-Oct-2011
Publisher: Associação Brasileira de Divulgação Científica
Citation: Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 10, p. 1013-1017, 2011.
Abstract: An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were characterized as SCCmec, mecA and PVL by PCR, pulsed-field gel electrophoresis (PFGE) profile and molecular sequence typing (MLST) genotyping. The isolates presented type IV SCCmec, and none proved to be positive for PVL. The isolates showed a PFGE profile similar to the pediatric clone. MLST genotyping demonstrated that the isolates belonged to clonal complex 5 (CC5), showing a new yqiL allele gene, resulting in a new sequence typing (ST) (1176). Our results showed that strains of MRSA carrying a new ST are emerging in community and nosocomial infections, including bacteremia, in São Paulo, Brazil.
ISSN: 0100-879X
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