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Title: Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid
Authors: Pastrelo, Maurcio Mercaldi [UNIFESP]
Dias Ribeiro, Carla Caroline [UNIFESP]
Duarte, Joselmo Willamys [UNIFESP]
Bioago Gollucke, Andrea Pitelli [UNIFESP]
Artigiani-Neto, Ricardo [UNIFESP]
Ribeiro, Daniel Araki [UNIFESP]
Miszputen, Sender Jankiel [UNIFESP]
Fujiyama Oshima, Celina Tizuko [UNIFESP]
Ribeiro Paiotti, Ana Paula [UNIFESP]
Keywords: Experimental colitis
apple extract
Issue Date: 2017
Publisher: Cellular & Molecular Biology Research Center
Citation: International Journal Of Molecular And Cellular Medicine. Babol, v. 6, n. 1, p. 38-49, 2017.
Abstract: Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1-Sham/saline solution, G2-CAE/control, G3-acetic acid/control, G4-curative-CAE treatment and G5-preventive-CAE treatment. Eight days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) via immunohistochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD.
ISSN: 2251-9637
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Appears in Collections:Artigo

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