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Title: Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
Authors: Carraro, Emerson [UNIFESP]
Pasternak, Jacyr [UNIFESP]
Perosa, Ana Helena Sitta [UNIFESP]
Siqueira, Itacy [UNIFESP]
Martino, Marines Dalla Vale [UNIFESP]
Hospital Albert Einstein Clinical Laboratory Microbiology Section
Universidade Federal de São Paulo (UNIFESP)
Keywords: cytomegalovirus
antigenemia assay
Issue Date: 1-Jun-2010
Publisher: Brazilian Society of Infectious Diseases
Citation: Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 14, n. 3, p. 322-324, 2010.
Abstract: Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results.
ISSN: 1413-8670
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