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dc.contributor.authorKarpel, Richard L.
dc.contributor.authorLiberato, Michelle da Silva
dc.contributor.authorCampeiro, Joana Darc [UNIFESP]
dc.contributor.authorBergeon, Lorna
dc.contributor.authorSzychowski, Brian
dc.contributor.authorButler, Andrew
dc.contributor.authorMarino, Giovanni
dc.contributor.authorCusic, Joelle F.
dc.contributor.authorOliveira, Lilian Caroline Goncalves de [UNIFESP]
dc.contributor.authorOliveira, Eduardo B.
dc.contributor.authorFarias, Marcelo Alexandre de
dc.contributor.authorPortugal, Rodrigo Villares
dc.contributor.authorAlves, Wendel Andrade
dc.contributor.authorDaniel, Marie-Christine
dc.contributor.authorHayashi, Mirian Akemi Furuie [UNIFESP]
dc.identifier.citationColloids And Surfaces B-Biointerfaces. Amsterdam, v. 163, p. 43313, 2018.
dc.description.abstractThis paper describes the development of a facile and environmentally friendly strategy for supporting crotamine on gold nanoparticles (GNPs). Our approach was based on the covalent binding interaction between the cell penetrating peptide crotamine, which is a snake venom polypeptide with preference to penetrate dividing cells, and a polyethylene glycol (PEG) ligand, which is a nontoxic, water-soluble and easily obtainable commercial polymer. Crotamine was derivatized with ortho-pyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-SVA) cross-linker to produce OPSS-PEG-crotamine as the surface modifier of GNP. OPSS-PEG-SVA can serve not only as a surface modifier, but also as a stabilizing agent for GNPs. The successful PEGylation of the nanoparticles was demonstrated using different physicochemical techniques, while the grafting densities of the PEG ligands and crotamine on the surface of the nanoparticles were estimated using a combination of electron microscopy and mass spectrometry analysis. In vitro assays confirmed the internalization of these GNPs, into living HeLa cells. The results described herein suggest that our approach may serve as a simple platform for the synthesis Of GNPs decorated with crotamine with well-defined morphologies and uniform dispersion, opening new roads for crotamine biomedical applications. (C) 2017 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipSao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo FAPESP)
dc.description.sponsorshipNational Council of Technological and Scientific Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq)
dc.description.sponsorshipNational Science Foundationx
dc.description.sponsorshipUMBC Designated Resarch Initiative Fund
dc.description.sponsorshipBrazil Scientific Mobility Program
dc.description.sponsorshipUndergraduate Research Award from UMBC
dc.description.sponsorshipNational Institutes of Health SIG grant
dc.publisherElsevier Science Bv
dc.relation.ispartofColloids And Surfaces B-Biointerfaces
dc.rightsAcesso restrito
dc.subjectSnake toxinen
dc.subjectGold nanoparticleen
dc.subjectPolyethylene glycolen
dc.subjectCell penetrating peptideen
dc.titleDesign and characterization of crotamine-functionalized gold nanoparticlesen
dc.description.affiliationUMBC, Dept Chem & Biochem, 1000 Hilltop Circle, Baltimore, MD 21250 USA
dc.description.affiliationUniv Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, EPM, Dept Biofis, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo USP RP, Dept Bioquim & Imunol, Ribeirao Preto, SP, Brazil
dc.description.affiliationCNPEM, Brazilian Nanotechnol Natl Lab LNNano, Campinas, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, EPM, Dept Biofis, Sao Paulo, SP, Brazil
dc.description.sponsorshipIDFAPESP: 2013/13392-4
dc.description.sponsorshipIDFAPESP: 2015/24018-1
dc.description.sponsorshipIDFAPESP: 2017/02413-1
dc.description.sponsorshipIDCNPq: 477760/2010-4
dc.description.sponsorshipIDCNPq: 557753/2010-4
dc.description.sponsorshipIDCNPq: 508113/2010-5
dc.description.sponsorshipIDCNPq: 311815/2012-0
dc.description.sponsorshipIDCNPq: 475739/2013-2
dc.description.sponsorshipIDCNPq: 302923/2015-2
dc.description.sponsorshipIDCNPq: 309337/2016-0
dc.description.sponsorshipIDNSF: CHE 1507462
dc.description.sponsorshipIDNIH: 1S10RR26870-1
dc.description.sourceWeb of Science
dc.citation.volumev. 163
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