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Title: Design and characterization of crotamine-functionalized gold nanoparticles
Authors: Karpel, Richard L.
Liberato, Michelle da Silva
Campeiro, Joana Darc [UNIFESP]
Bergeon, Lorna
Szychowski, Brian
Butler, Andrew
Marino, Giovanni
Cusic, Joelle F.
Oliveira, Lilian Caroline Goncalves de [UNIFESP]
Oliveira, Eduardo B.
Farias, Marcelo Alexandre de
Portugal, Rodrigo Villares
Alves, Wendel Andrade
Daniel, Marie-Christine
Hayashi, Mirian Akemi Furuie [UNIFESP]
Keywords: Snake toxin
Gold nanoparticle
Polyethylene glycol
Cell penetrating peptide
Issue Date: 2018
Publisher: Elsevier Science Bv
Citation: Colloids And Surfaces B-Biointerfaces. Amsterdam, v. 163, p. 43313, 2018.
Abstract: This paper describes the development of a facile and environmentally friendly strategy for supporting crotamine on gold nanoparticles (GNPs). Our approach was based on the covalent binding interaction between the cell penetrating peptide crotamine, which is a snake venom polypeptide with preference to penetrate dividing cells, and a polyethylene glycol (PEG) ligand, which is a nontoxic, water-soluble and easily obtainable commercial polymer. Crotamine was derivatized with ortho-pyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-SVA) cross-linker to produce OPSS-PEG-crotamine as the surface modifier of GNP. OPSS-PEG-SVA can serve not only as a surface modifier, but also as a stabilizing agent for GNPs. The successful PEGylation of the nanoparticles was demonstrated using different physicochemical techniques, while the grafting densities of the PEG ligands and crotamine on the surface of the nanoparticles were estimated using a combination of electron microscopy and mass spectrometry analysis. In vitro assays confirmed the internalization of these GNPs, into living HeLa cells. The results described herein suggest that our approach may serve as a simple platform for the synthesis Of GNPs decorated with crotamine with well-defined morphologies and uniform dispersion, opening new roads for crotamine biomedical applications. (C) 2017 Elsevier B.V. All rights reserved.
ISSN: 0927-7765
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