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Title: Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
Authors: Batista Júnior, João
Berzaghi, Rodrigo [UNIFESP]
Arnaud, Adelita Duanra Medeiros do Monte
Fontes, Cor Jesus Fernandes
Camargo, Zoilo Pires de [UNIFESP]
Hahn, Rosane Christine
Universidade Federal de Mato Grosso Faculdade de Ciências Médicas Laboratório de Micologia
Universidade Federal de São Paulo (UNIFESP)
Universidade de Cuiabá Hospital Geral Universitário Laboratório de Micologia
Keywords: Paracoccidioides brasiliensis
simultaneous infection
Issue Date: 1-Feb-2010
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 1, p. 62-65, 2010.
Abstract: This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
ISSN: 0074-0276
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