Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/5443
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dc.contributor.authorTorres, Rogil José de Almeida [UNIFESP]
dc.contributor.authorMaia, Maurício [UNIFESP]
dc.contributor.authorPrécoma, Dalton Bertolin
dc.contributor.authorNoronha, Lucia
dc.contributor.authorLuchini, Andréa
dc.contributor.authorPrécoma, Leonardo Brandão
dc.contributor.authorSouza, Greyce Kelly de
dc.contributor.authorMuccioli, Cristina [UNIFESP]
dc.date.accessioned2015-06-14T13:41:21Z
dc.date.available2015-06-14T13:41:21Z
dc.date.issued2009-12-01
dc.identifierhttp://dx.doi.org/10.1590/S0004-27492009000600010
dc.identifier.citationArquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 72, n. 6, p. 793-798, 2009.
dc.identifier.issn0004-2749
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5443
dc.description.abstractPURPOSE: The aim of this study is to demonstrate the early changes of the sensory retina induced by hypercholesterolemia in an experimental model. METHODS: New Zealand rabbits were divided into two groups: CG (Control Group) was fed a normal diet for 6 weeks. G1 was initially fed a 1% cholesterol diet for two weeks and from the 14th day on a 0.5% cholesterol diet until the 42nd day. The eyes underwent an immunohistochemical analysis with monoclonal antibodies anti-calretinin and anti-glial fibrillary acidic protein (GFAP). RESULTS: G1 cells and cell elements presented significant immunoreactivity to anti-calretinin. No immunoreactivity to anti-glial fibrillary acidic protein was observed in both groups. CONCLUSION: This study has shown that a hypercholesterolemic diet may induce early changes in the sensory retina in rabbits. The anti-calretinin monoclonal antibody was able to reveal calcium accumulation inside the nerve cells.en
dc.description.abstractOBJETIVO: O objetivo deste estudo é demonstrar experimentalmente as alterações precoces da retina sensorial induzidas pela hipercolesterolemia. MÉTODOS: Coelhos New Zealand foram organizados em dois grupos: GC (grupo controle), composto por 6 coelhos (6 olhos), recebeu dieta normal por 6 semanas; G1, composto por 12 coelhos (12 olhos), tratado previamente com ração colesterol a 1% (Sigma-Aldrich) por 2 semanas e a partir do 14º dia com ração colesterol a 0,5% (Sigma-Aldrich). Os olhos foram submetidos à análise imunohistoquímica com os anticorpos monoclonais anticalretinina e anti-glial fibrillary acidic protein (GFAP). RESULTADOS: G1 apresentou maior número de células e elementos celulares imunoreativos a anticalretinina que o GC, com relevância estatística. GFAP foi negativo em ambos os grupos. CONCLUSÃO: Este estudo demonstrou que a dieta hipercolesterolêmica pode induzir alterações precoces na retina sensorial em coelhos. O anticorpo monoclonal anticalretinina foi capaz de revelar o acúmulo de cálcio dentro das células neuronais retiniana.pt
dc.format.extent793-798
dc.language.isoeng
dc.publisherConselho Brasileiro de Oftalmologia
dc.relation.ispartofArquivos Brasileiros de Oftalmologia
dc.rightsAcesso aberto
dc.subjectRetinaen
dc.subjectModels, animalen
dc.subjectCalcium-binding proteinsen
dc.subjectCholesterolen
dc.subjectAnoxiaen
dc.subjectImmunohistochemistryen
dc.subjectRabbitsen
dc.subjectRetinapt
dc.subjectModelos animaispt
dc.subjectProteínas de ligação do cálciopt
dc.subjectColesterolpt
dc.subjectAnoxiapt
dc.subjectImuno-histoquímicapt
dc.subjectCoelhospt
dc.titleEvaluation of early abnormalities of the sensory retina in a hypercholesterolemia experimental model: an immunohistochemical studyen
dc.title.alternativeAvaliação das anormalidades precoces da retina sensorial em modelo experimental de hipercolesterolemia: estudo imunohistoquímicopt
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHospital de Olhos Oeste Paulista Vitreoretinal Surgery Service
dc.contributor.institutionPontifícia Universidade Católica do Paraná - PUCPR Cardiology Department
dc.contributor.institutionPUCPR Pathology Department
dc.contributor.institutionCentro Oftalmológico de Curitiba
dc.contributor.institutionPUCPR
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) - UNIFESP Ophthalmology Departmen
dc.description.affiliationHospital de Olhos Oeste Paulista Vitreoretinal Surgery Service
dc.description.affiliationPontifícia Universidade Católica do Paraná - PUCPR Cardiology Department
dc.description.affiliationPUCPR Pathology Department
dc.description.affiliationCentro Oftalmológico de Curitiba
dc.description.affiliationPUCPR
dc.description.affiliationUNIFESP Ophthalmology Department
dc.description.affiliationUnifespUNIFESP, Ophthalmology Departmen
dc.description.affiliationUnifespUNIFESP, Ophthalmology Department
dc.identifier.fileS0004-27492009000600010.pdf
dc.identifier.scieloS0004-27492009000600010
dc.identifier.doi10.1590/S0004-27492009000600010
dc.description.sourceSciELO
dc.identifier.wosWOS:000274591700010
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