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Title: The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
Authors: Santos, Marcia Regina Machado [UNIFESP]
Cano, Maria I [UNIFESP]
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J
Ramirez, José Luis
Brandão, Adeílton
Degrave, Wim M
Franco da Silveira, José [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular
Universidad Central de Venezuela
Instituto Oswaldo Cruz
Keywords: Trypanosoma cruzi
clone CL
genome project
molecular karyotype
chromosome mapping
genetic map
Issue Date: 1-Nov-1997
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.
Abstract: By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes
ISSN: 0074-0276
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