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dc.contributor.authorde Camargo, Tarsila Mendes
dc.contributor.authorde Freitas, Elisangela Oliveira
dc.contributor.authorGimenez, Alba Marina [UNIFESP]
dc.contributor.authorLima, Luciana Chagas
dc.contributor.authorCaramico, Karina de Almeida
dc.contributor.authorFrancoso, Katia Sanches
dc.contributor.authorBruna-Romero, Oscar
dc.contributor.authorAndolina, Chiara
dc.contributor.authorNosten, Francois
dc.contributor.authorRenia, Laurent
dc.contributor.authorErtl, Hildegund C. J.
dc.contributor.authorNussenzweig, Ruth S.
dc.contributor.authorNussenzweig, Victor
dc.contributor.authorRodrigues, Mauricio M. [UNIFESP]
dc.contributor.authorReyes-Sandoval, Arturo
dc.contributor.authorSoares, Irene S.
dc.identifier.citationScientific Reports. London, v. 8, p. -, 2018.-
dc.description.abstractVaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-All(FL)) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-All(CT)) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria.en
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia de Vacinas (INCTV)
dc.description.sponsorshipFAPESP fellowships
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) fellowship
dc.description.sponsorshipMedical Research Council, MRC
dc.description.sponsorshipWellcome Trust
dc.description.sponsorshipWellcome Trust Mahidol University Oxford Tropical Medicine Research Programme - Wellcome Trust
dc.description.sponsorshipSingapore Immunology Network (SIgN)
dc.description.sponsorshipHorizontal Programme on Infectious Diseases under Agency for Science, Technology and Research (A*STAR, Singapore)
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsAcesso aberto
dc.titlePrime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challengeen
dc.description.affiliationUniv Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Oxford, Nuffield Dept Med, Jenner Inst, Henry Wellcome Bldg Mol Physiol,Roosevelt Dr, Oxford, England
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Ctr Cellular & Mol Therapy, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Santa Catarina, Dept Microbiol & Immunol, Florianopolis, SC, Brazil
dc.description.affiliationMahidol Univ, Fac Trop Med, Shoklo Malaria Res Unit, Mahidol Oxford Trop Med Res Unit, Mae Sot, Thailand
dc.description.affiliationCtr Trop Med & Global Hlth, Nuffield Dept Med Res Bldg, Oxford, England
dc.description.affiliationAgcy Sci Technol & Res, Singapore Immunol Network, Biopolis, Singapore, Singapore
dc.description.affiliationWistar Inst Anat & Biol, 3601 Spruce St, Philadelphia, PA 19104 USA
dc.description.affiliationNYU, Sch Med, Dept Pathol, Michael Heidelberger Div, New York, NY USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Ctr Cellular & Mol Therapy, Sao Paulo, SP, Brazil
dc.description.sponsorshipIDFAPESP: 2012/13032-5
dc.description.sponsorshipIDFAPESP fellowships: 2014/18102-7, 2009/15099-7
dc.description.sponsorshipIDMedical Research Council, MRC: MR/N019008/1
dc.description.sponsorshipIDWellcome Trust: 089179
dc.description.sourceWeb of Science
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