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dc.contributor.authorRamirez, Marcel I.
dc.contributor.authorAmorim, Maria G.
dc.contributor.authorGadelha, Catarina
dc.contributor.authorMilic, Ivana
dc.contributor.authorWelsh, Joshua A.
dc.contributor.authorFreitas, Vanessa M.
dc.contributor.authorNawaz, Muhammad
dc.contributor.authorAkbar, Naveed
dc.contributor.authorCouch, Yvonne
dc.contributor.authorMakin, Laura
dc.contributor.authorCooke, Fiona
dc.contributor.authorVettore, Andre L. [UNIFESP]
dc.contributor.authorBatista, Patricia X. [UNIFESP]
dc.contributor.authorFreezor, Roberta
dc.contributor.authorPezuk, Julia A.
dc.contributor.authorRosa-Fernandes, Livia
dc.contributor.authorCarreira, Ana Claudia O.
dc.contributor.authorDevitt, Andrew
dc.contributor.authorJacobs, Laura
dc.contributor.authorSilva, Israel T.
dc.contributor.authorCoakley, Gillian
dc.contributor.authorNunes, Diana N.
dc.contributor.authorCarter, Dave
dc.contributor.authorPalmisano, Giuseppe
dc.contributor.authorDias-Neto, Emmanuel
dc.identifier.citationNanoscale. Cambridge, v. 10, n. 3, p. 881-906, 2018.-
dc.description.abstractExtracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.en
dc.description.sponsorshipBritish Council/Newton Fund, Brazil [203855854]
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil [2012/24574-3, 2015/50257-3, 2014/26897-0, 2014/06863-3]
dc.description.sponsorshipAssociacao Beneficente Alzira Denise Hertzog Silva (ABADHS), Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil [441878/2014-8, 202077/2015-2]
dc.description.sponsorshipRicerca Finalizzata from the Ministero della Salute, Italy [172/GR-2011-02350301]
dc.description.sponsorshipScience without Borders/CAPES, Brazil [BEX 7057/15-6]
dc.publisherRoyal Soc Chemistry
dc.rightsAcesso aberto
dc.titleTechnical challenges of working with extracellular vesiclesen
dc.description.affiliationFundacao Oswaldo Cruz, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Fed Parana, Curitiba, Parana, Brazil
dc.description.affiliationAC Camargo Canc Ctr, CIPE, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Nottingham, Sch Life Sci, Nottingham, England
dc.description.affiliationAston Univ, Sch Life & Hlth Sci, Birmingham, W Midlands, England
dc.description.affiliationUniv Southampton, Southampton, England
dc.description.affiliationUniv Sao Paulo, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Gothenburg, Gothenburg, Sweden
dc.description.affiliationUniv Oxford, Div Cardiovasc Med, Oxford, England
dc.description.affiliationUniv Oxford, Acute Stroke Programme, RDM Investigat Med, Oxford, England
dc.description.affiliationUniv Oxford, Sir William Dunn Sch Pathol, Oxford, England
dc.description.affiliationUniv St Andrews, St Andrews, Fife, Scotland
dc.description.affiliationUniv Fed Sao Paulo, Campus Diadema, Diadema, Brazil
dc.description.affiliationLondon Metropolitan Univ, London, England
dc.description.affiliationUniv Anhanguera Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUniv Southern Denmark, Odense, Denmark
dc.description.affiliationTech Univ Munich, Munich, Germany
dc.description.affiliationUniv Glasgow, Inst Infect Immun & Inflammat, Glasgow, Lanark, Scotland
dc.description.affiliationOxford Brookes Univ, Oxford, England
dc.description.affiliationFdn Santa Lucia, IRCCS, Rome, Italy
dc.description.affiliationUnifespUniv Fed Sao Paulo, Campus Diadema, Diadema, Brazil
dc.description.sponsorshipIDBritish Council/Newton Fund, Brazil:203855854
dc.description.sponsorshipIDFAPESP: 2012/24574-3, 2015/50257-3, 2014/26897-0, 2014/06863-3
dc.description.sponsorshipIDConselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil : 441878/2014-8, 202077/2015-2
dc.description.sponsorshipIDRicerca Finalizzata from the Ministero della Salute, Italy: 172/GR-2011-02350301
dc.description.sponsorshipIDScience without Borders/CAPES, Brazil: BEX 7057/15-6
dc.description.sourceWeb of Science
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