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Title: Viability of primary human pigment epithelium cells and muller-glia cells after intravitreal ziv-aflibercept and aflibercept
Authors: de Andrade, Gabriel Costa [UNIFESP]
Wertheimer, Christian
Eibl, Kirsten
Wolf, Armin
Kampik, Anselm
Rodrigues, Eduardo Buchele [UNIFESP]
Farah, Michel Eid [UNIFESP]
Haritoglou, Christos [UNIFESP]
Keywords: Ziv-Aflibercept
ToxicityMacular Degeneration
Issue Date: 2016
Publisher: Taylor & Francis Inc
Citation: Ophthalmologica. Basel, v. 236, n. 4, p. 223-227, 2016.
Abstract: Purpose: The aim of this study was to access the safety profiles of 2 fusion proteins with anti-vascular endothelial growth factor action (ziv-aflibercept and aflibercept) on retinal pigment epithelium cells and Muller-Glia cells in culture by assessing cell viability post drug exposure. Methods: Primary human retinal pigment epithelium cells (pRPE) and Muller-Glia cells (Mio-M1) were exposed to the clinical standardized concentrations of ziv-aflibercept (25 mg/mL) and aflibercept (40 mg/mL). Progressively higher concentrations of NaCI (300, 500, 1,000, 1,500, 2,000, 5,000, and 10,000 mosm/kg) were also applied to cells to assess the possibility of potentiating hyperosmotic cytotoxity effect. The study was applied to measure pRPE and Mio-M1 viability by a tetrazolium dye-reduction assay (XTT). Results: Cell viability of both pRPE and Mio-M1 presented no significant changes after exposure of ziv-aflibercept and aflibercept. Progressive NaCI concentrations did not significantly alter cell viability. The exposure to the negative control of 75 mu L/mL of dimethyl sulfoxide showed significant reduction in cell viability. Conclusions: At clinical doses, neither ziv-aflibercept nor aflibercept caused any significant reduction in cell viability in vitro. Furthermore, injection solutions of NaCI with higher osmolality caused no significant reduction in cell viability. (C) 2017 S. Karger AG, Basel
ISSN: 0030-3755
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