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dc.contributor.authorde Andrade, Gabriel Costa [UNIFESP]
dc.contributor.authorWertheimer, Christian
dc.contributor.authorEibl, Kirsten
dc.contributor.authorWolf, Armin
dc.contributor.authorKampik, Anselm
dc.contributor.authorRodrigues, Eduardo Buchele [UNIFESP]
dc.contributor.authorFarah, Michel Eid [UNIFESP]
dc.contributor.authorHaritoglou, Christos [UNIFESP]
dc.identifier.citationOphthalmologica. Basel, v. 236, n. 4, p. 223-227, 2016.
dc.description.abstractPurpose: The aim of this study was to access the safety profiles of 2 fusion proteins with anti-vascular endothelial growth factor action (ziv-aflibercept and aflibercept) on retinal pigment epithelium cells and Muller-Glia cells in culture by assessing cell viability post drug exposure. Methods: Primary human retinal pigment epithelium cells (pRPE) and Muller-Glia cells (Mio-M1) were exposed to the clinical standardized concentrations of ziv-aflibercept (25 mg/mL) and aflibercept (40 mg/mL). Progressively higher concentrations of NaCI (300, 500, 1,000, 1,500, 2,000, 5,000, and 10,000 mosm/kg) were also applied to cells to assess the possibility of potentiating hyperosmotic cytotoxity effect. The study was applied to measure pRPE and Mio-M1 viability by a tetrazolium dye-reduction assay (XTT). Results: Cell viability of both pRPE and Mio-M1 presented no significant changes after exposure of ziv-aflibercept and aflibercept. Progressive NaCI concentrations did not significantly alter cell viability. The exposure to the negative control of 75 mu L/mL of dimethyl sulfoxide showed significant reduction in cell viability. Conclusions: At clinical doses, neither ziv-aflibercept nor aflibercept caused any significant reduction in cell viability in vitro. Furthermore, injection solutions of NaCI with higher osmolality caused no significant reduction in cell viability. (C) 2017 S. Karger AG, Baselen
dc.publisherTaylor & Francis Inc
dc.rightsAcesso restrito
dc.subjectToxicityMacular Degenerationen
dc.titleViability of primary human pigment epithelium cells and muller-glia cells after intravitreal ziv-aflibercept and aflibercepten
dc.description.affiliationDepartment of Ophthalmology, Federal University of São Paulo, Botucatu St 821, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationDepartment of Ophthalmology, Ludwig-Maximilian-University, Munich, Germany
dc.description.affiliationUnifespDepartment of Ophthalmology, Federal University of São Paulo, Botucatu St 821, BR-04023062 Sao Paulo, Brazil
dc.description.sourceWeb of Science
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