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Title: Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
Authors: Figueiredo, Thais Pandini
de Lucas, Rosymar Coutinho
Cazzaniga, Rodrigo Anselmo
Franca, Carolina Nunes [UNIFESP]
Segato, Fernando
Taglialegna, Rafael
Maffei, Cláudia Maria Leite
Keywords: Cryptococcus Neoformans
Antifungal Agents
Mating Type
Species Complex
Molecular Characterization
Var. Gattii
Issue Date: 2016
Publisher: Wiley
Citation: Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.
Abstract: Cryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared
besides, the studied strains did not present a significant increase in resistance to antifungal agents. In addition, the homogeneity of the molecular types and detection of the mating types suggested a low possibility of crossing among the strains.
ISSN: 0036-4665
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Appears in Collections:Artigo

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