Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/45134
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dc.contributor.authorDias Neto, Emmanuel
dc.contributor.authorCorrea, Ricardo Garcia
dc.contributor.authorVerjovski-Almeida, Sergio
dc.contributor.authorBriones, Marcelo Ribeiro da Silva [UNIFESP]
dc.contributor.authorNagai, Maria Aparecida
dc.contributor.authorSilva Junior, Wilson da
dc.contributor.authorZago, Marco Antonio
dc.contributor.authorBordin, Silvana
dc.contributor.authorCosta, Fernando Ferreira [UNIFESP]
dc.contributor.authorGoldman, Gustavo Henrique
dc.contributor.authorCarvalho, Alex Fiorini de
dc.contributor.authorMatsukuma, Adriana
dc.contributor.authorBaia, Gilson Soares
dc.contributor.authorSimpson, David H.
dc.contributor.authorBrunstein, Adriana [UNIFESP]
dc.contributor.authorOliveira, Paulo Sergio Lopes de
dc.contributor.authorBucher, Philipp
dc.contributor.authorJongeneel, C. Victor
dc.contributor.authorO'Hare, Michael J.
dc.contributor.authorSoares, Fernando
dc.contributor.authorBrentani, Ricardo Renzo [UNIFESP]
dc.contributor.authorReis, Luis Fernando Lima
dc.contributor.authorSouza, Sandro José de
dc.contributor.authorSimpson, Andrew JG
dc.date.accessioned2018-06-18T11:27:13Z-
dc.date.available2018-06-18T11:27:13Z-
dc.date.issued2000-03-28
dc.identifierhttp://dx.doi.org/10.1073/pnas.97.7.3491
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America. Washington: Natl Acad Sciences, v. 97, n. 7, p. 3491-3496, 2000.
dc.identifier.issn0027-8424
dc.identifier.urihttp://repositorio.unifesp.br/11600/45134-
dc.description.abstractTheoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. systematic, high-throughput sequencing of such products would result in an expressed sequence tag (EST) database consisting of central, generally coding regions of expressed genes. Such a database would add significant value to existing public EST databases, which consist mostly of sequences derived from the extremities of cDNAs, and facilitate the construction of contigs of transcript sequences. We tested our predictions, creating a database of 10,000 sequences from human breast tumors. The data confirmed the central distribution of the sequences, the significant normalization of the sequence population, the frequent extension of contigs composed of existing human ESTs, and the identification of a series of potentially important homologues of known genes. This approach should make a significant contribution to the early identification of important human genes, the deciphering of the draft human genome sequence currently being compiled, and the shotgun sequencing of the human transcriptome.en
dc.format.extent3491-3496
dc.language.isoeng
dc.publisherNatl Acad Sciences
dc.relation.ispartofProceedings Of The National Academy Of Sciences Of The United States Of America
dc.rightsAcesso aberto
dc.titleShotgun sequencing of the human transcriptome with ORF expressed sequence tagsen
dc.typeArtigo
dc.contributor.institutionLudwig Inst Canc Res
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Med Ribeirao Preto
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionFac Ciencias Farmaceut Ribeirao Preto
dc.contributor.institutionSwiss Inst Bioinformat
dc.contributor.institutionSwiss Inst Expt Canc Res
dc.contributor.institutionUCL
dc.contributor.institutionHosp AC Camargo Fund Antonio Prudente
dc.description.affiliationLudwig Inst Canc Res, BR-01509010 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med, Dept Radiol, BR-01296903 Sao Paulo, Brazil
dc.description.affiliationFac Med Ribeirao Preto, Dept Clin Med, BR-14049900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Hemoctr, BR-13089970 Sao Paulo, Brazil
dc.description.affiliationFac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14049900 Sao Paulo, Brazil
dc.description.affiliationSwiss Inst Bioinformat, CH-1066 Epalinges, Switzerland
dc.description.affiliationSwiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland
dc.description.affiliationLudwig Inst Canc Res, Off Informat Technol, CH-1066 Epalinges, Switzerland
dc.description.affiliationUCL, Ludwig Inst Canc Res, London W1P 7LD, England
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, BR-01509010 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil
dc.identifier.doi10.1073/pnas.97.7.3491
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000086195200096
Appears in Collections:Artigo

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