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|Title:||The clinical spectrum of antinuclear antibodies associated with the nuclear dense fine speckled immunofluorescence pattern|
Viana, Vilma ST
Leon, Elaine R.
Bonfa, Eloisa SDO
Andrade, Luís EC
Leser, Paulo G.
Fleury Res Inst
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
|Publisher:||J Rheumatol Publ Co|
|Citation:||Journal Of Rheumatology. Toronto: J Rheumatol Publ Co, v. 32, n. 11, p. 2144-2149, 2005.|
|Abstract:||Objective. Autoantibodies to lens epithelium-derived growth factor (LEDGF) depict a distinctive nuclear dense fine speckled (DFS) pattern in the indirect immunofluorescence antinuclear antibody assay (IIF-ANA). Definition of the clinical spectrum associated with anti-LEDGF antibodies has been evolving over the last decade. We investigated the frequency, clinical spectrum, and immunologic specificity of the DFS pattern in a general clinical laboratory routine.Methods. All serum samples entered for IIF-ANA determination within a 2 year period were examined for the DFS pattern. Positive samples with consistent clinical information were studied further by IIF with isotype-specific conjugate and immunoblot analysis.Results. Among 13,641 ANA-positive samples, 5081 (37%) presented the DFS pattern. Within a 6 month nested period, there were 650 samples with DFS pattern, and consistent clinical data were available for 81 of these. DFS reactivity was mainly due to IgG. Most samples (86%) presented titer >= 1/640. Eighty of the 81 DFS samples reacted with a 75 kDa band that comigrated with the band elicited by the standard anti-LEDGF serum. Antibodies that were affinity-purified from the 75 kDa band reproduced the DFS pattern on IIF-ANA. The clinical spectrum associated with DFS reactivity included autoimmune diseases (39%) and an array of nonautoimmune conditions (61%). Among the autoimmune patients, over half presented evidence of autoimmune thyroiditis.Conclusion. Anti-LEDGF/p75 antibodies are a common finding among ANA-positive individuals with no evidence of rheumatic autoimmune disease, and should be regarded as a low specificity finding even when in moderate or high titer.|
|Appears in Collections:||Artigo|
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