Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/44384
Title: Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)
Authors: Oliani, Sonia M.
Freymuller, Edna [UNIFESP]
Takahashi, Helio K. [UNIFESP]
Straus, Anita H. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
UNESP
Keywords: mast cells
heparin
granules
immunolabeling
monoclonal antibody
electron microscopy
Issue Date: 1-Feb-1997
Publisher: Histochemical Soc Inc
Citation: Journal Of Histochemistry & Cytochemistry. Seattle: Histochemical Soc Inc, v. 45, n. 2, p. 231-235, 1997.
Abstract: We performed immunogold labeling with an ST-1 monoclonal antibody (IgM), specific for intact heparin, to define the subcellular localization of heparin in mast cells. Rat peritoneal mast cells were fixed by a modified Karnovsky method and embedded in Araldite. Ultrathin sections were first treated with sodium periodate and then sequentially incubated with MAb ST-1, rabbit anti-mouse IgM, and protein A-gold. By transmission electron microscopy, gold particles were localized inside cytoplasmic granules of peritoneal mast cells. In contrast, with the same procedure, no labeling was observed in mast cells from rat intestinal mucosa. Control sections of rat peritoneal or intestinal mucosa mast Mast cells cells treated with an irrelevant MAb (IgM) did not show any labeling. Treatment with nitrous Heparin acid abolished the reactivity of MAb ST-1 with peritoneal mast cells. These results Granules show that different mast cells can be identified regarding their heparin content by immunochemical procedures using MAb ST-1.
URI: http://repositorio.unifesp.br/11600/44384
ISSN: 0022-1554
Other Identifiers: http://dx.doi.org/10.1177/002215549704500208
Appears in Collections:Artigo
Artigo

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.