Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/44341
Title: Bioengineered teeth from cultured rat tooth bud cells
Authors: Duailibi, M. T.
Duailibi, S. E.
Young, C. S.
Bartlett, J. D.
Vacanti, J. P.
Yelick, P. C.
Forsyth Inst
Harvard Univ
Universidade Federal de São Paulo (UNIFESP)
Massachusetts Gen Hosp
Keywords: tooth tissue engineering
dental stem cells
Issue Date: 1-Jul-2004
Publisher: Int Amer Assoc Dental Researchi A D R/a A D R
Citation: Journal Of Dental Research. Alexandria: Int Amer Assoc Dental Researchi A D R/a A D R, v. 83, n. 7, p. 523-528, 2004.
Abstract: The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
URI: http://repositorio.unifesp.br/11600/44341
ISSN: 0022-0345
Other Identifiers: http://dx.doi.org/10.1177/154405910408300703
Appears in Collections:Artigo
Artigo

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