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Title: Evaluation of recombinant antigens for serodiagnosis of Chagas' disease in south and central America
Authors: Umezawa, Eufrosina Setsu
Bastos, S. F.
Camargo, M. E.
Yamauchi, Lucy Megumi [UNIFESP]
Santos, Marcia R. [UNIFESP]
Gonzalez, A.
Zingales, B.
Levin, M. J.
Sousa, O.
Rangel-Aldao, R.
Silveira, Jose Franco da [UNIFESP]
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Inst Invest Engn Genet & Biol Mol
Univ Panama
Univ Simon Bolivar
Issue Date: 1-May-1999
Publisher: Amer Soc Microbiology
Citation: Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 37, n. 5, p. 1554-1560, 1999.
Abstract: The commercially available diagnostic tests for Chagas' disease employ whole extracts or semipurified fractions of Trypanosoma cruzi epimastigotes. Considerable variation in the reproducibility and ri liability of these tests has been reported by different research laboratories, mainly due to cross-reactivity with other pathogens and standardization of the reagents. The use of recombinant antigens for the serodiagnosis of Chagas' disease is recommended to increase the sensitivity and specificity of serological tests. Expressed in Escherichia call, as fusion products with glutathione S-transferase. sir T. cruzi recombinant antigens (H49, JL7, A13, B13, JL8, and 1F8) were evaluated in an enzyme-linked immunosorbent assay for Chagas' disease, The study was carried out with a panel of 541 serum samples of chagasic and nonchagasic patients from nine countries of Latin America (Argentina, Bolivia, Brazil, Chile, Colombia, El Salvador, Guatemala, I Honduras, and Venezuela). The optimal concentration of each recombinant antigen for coating of plates was determined with the help of 84% labelled recombinant proteins. While the specificity of the epimastigote antigen was 84% because of false positives from leishmaniasis cases, for the recombinant antigens it varied from 96.2 to 99.6%. Recombinant antigens reacted with 79 to 100% of serum samples from chronic chagasic patients. In this way, it is proposed that a mixture of a few T. cruzi recombinant antigens should he employ ed in a diagnostic kit to minimize individual variation and promote high sensitivity in the diagnosis of Chagas' disease.
ISSN: 0095-1137
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