Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/44293
Title: COMPARISON OF E-TEST AND NATIONAL-COMMITTEE-FOR-CLINICAL-LABORATORY-STANDARDS BROTH MACRODILUTION METHOD FOR AZOLE ANTIFUNGAL SUSCEPTIBILITY TESTING
Authors: Colombo, Arnaldo Lopes [UNIFESP]
Barchiesi, F.
Mcgough, D. A.
Rinaldi, M. G.
UNIV TEXAS
AUDIE L MURPHY MEM VET ADM MED CTR
UNIV ANCONA
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Mar-1995
Publisher: Amer Soc Microbiology
Citation: Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 33, n. 3, p. 535-540, 1995.
Abstract: The use of Etest strips for antimicrobial susceptibility testing is a new and promising method with broad applications in microbiology. Since these strips contain a predefined continuous gradient of a drug, it is possible to obtain a reproducible, quantitative MIC reading. We performed a prospective and double-blinded study to compare the Etest and National Committee for Clinical Laboratory Standards (Villanova, Pa.) broth macrodilution methods for determining the MICs of fluconazole, itraconazole, and ketoconazole for 100 clinical isolates (25 Candida albicans, 25 Cryptococcus neofarmans var, neoformans, 20 Torulopsis [Candida] glabrata, 15 Candida tropicalis, and 15 Candida parapsilosis). The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-P guidelines. Despite differences between results for some species-drug combinations, Etest and macrobroth MICs were, in general, in good agreement. The MIC agreement rates for the two methods, within +/-1 dilution, were 71% for ketoconazole, 80% for fluconazole, and 84% for itraconazole. According to our data, Etest has potential utility as an alternative method.
URI: http://repositorio.unifesp.br/11600/44293
ISSN: 0095-1137
Other Identifiers: http://jcm.asm.org/content/33/3/535
Appears in Collections:Artigo

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