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https://repositorio.unifesp.br/handle/11600/44287| Title: | IDENTIFICATION AND DETECTION OF TRYPANOSOMA-CRUZI BY USING A DNA AMPLIFICATION FINGERPRINT OBTAINED FROM THE RIBOSOMAL INTERGENIC SPACER |
| Authors: | Gonzalez, N. Galindo, I Guevara, P. Novak, Estela Maria [UNIFESP] Scorza, J. V. Anez, N. Silveira, Jose Franco da [UNIFESP] Ramirez, J. L. UNIV CENT VENEZUELA GERENCIA NACL BIOTECNOL FAC CIENCIAS MERIDA NUCL UNIV RAFAEL RANGEL UNIV LOS ANDES Universidade Federal de São Paulo (UNIFESP) |
| Issue Date: | 1-Jan-1994 |
| Publisher: | Amer Soc Microbiology |
| Citation: | Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 32, n. 1, p. 153-158, 1994. |
| Abstract: | We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produces highly polymorphic DNA band patterns for different strains of Trypanosoma cruzi. By labeling the PCR products with digoxigenin and by chemiluminescence detection, we improved the assay sensitivity by three orders of magnitude to get T. cruzi strain fingerprints in feces of the trypanosome-infected triatomine bug vector. We also developed a capture assay for the digoxigenin-labeled PCR products that allowed us to detect T. cruzi in triatomine bug vector feces and in human serum samples with a solid support. |
| URI: | http://repositorio.unifesp.br/11600/44287 |
| ISSN: | 0095-1137 |
| Other Identifiers: | http://jcm.asm.org/content/32/1/153 |
| Appears in Collections: | Artigo |
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