Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/44287
Title: IDENTIFICATION AND DETECTION OF TRYPANOSOMA-CRUZI BY USING A DNA AMPLIFICATION FINGERPRINT OBTAINED FROM THE RIBOSOMAL INTERGENIC SPACER
Authors: Gonzalez, N.
Galindo, I
Guevara, P.
Novak, Estela Maria [UNIFESP]
Scorza, J. V.
Anez, N.
Silveira, Jose Franco da [UNIFESP]
Ramirez, J. L.
UNIV CENT VENEZUELA
GERENCIA NACL BIOTECNOL
FAC CIENCIAS MERIDA
NUCL UNIV RAFAEL RANGEL
UNIV LOS ANDES
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Jan-1994
Publisher: Amer Soc Microbiology
Citation: Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 32, n. 1, p. 153-158, 1994.
Abstract: We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produces highly polymorphic DNA band patterns for different strains of Trypanosoma cruzi. By labeling the PCR products with digoxigenin and by chemiluminescence detection, we improved the assay sensitivity by three orders of magnitude to get T. cruzi strain fingerprints in feces of the trypanosome-infected triatomine bug vector. We also developed a capture assay for the digoxigenin-labeled PCR products that allowed us to detect T. cruzi in triatomine bug vector feces and in human serum samples with a solid support.
URI: http://repositorio.unifesp.br/11600/44287
ISSN: 0095-1137
Other Identifiers: http://jcm.asm.org/content/32/1/153
Appears in Collections:Artigo

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