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Title: Segment-specific decrease of both catecholamine concentration and acetylcholinesterase activity are accompanied by nerve refinement in the rat cauda epididymis during sexual maturation
Authors: Silva, A. M.
Queiroz, Daniel Barboza Cava [UNIFESP]
Neto, EFC
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
Godinho, Rosely Oliveira [UNIFESP]
Porto, Catarina Segreti [UNIFESP]
Gutierrez-Ospina, G.
Avellar, Maria Christina Werneck [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Natl Autonomous Univ Mexico
Keywords: autonomic innervation
male reproductive tract
Issue Date: 1-May-2002
Publisher: Amer Soc Andrology, Inc
Citation: Journal Of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 23, n. 3, p. 374-383, 2002.
Abstract: In the present work, histochemical and biochemical studies were conducted to analyze changes in the pattern of autonomic innervation during sexual maturation, using the rat epididymis as a model. Glyoxylic acid histochemistry and immunohistochemical studies against dopamine beta-hydroxylase (DPH) and acetylcholinesterase (AChE) indicated a reduction in the amount of catecholaminergic and AChE-positive neurons, fibers, and puncta detected in the cauda epididymis of adult rats (120 days old), when compared to immature (40 days) and young adult (60 days) animals. No obvious age-related variations were detected in the few catecholaminergic and AChE-positive fibers and puncta present in the caput region. AChE-positive fibers were found sorting out among epithelial cells and ending free upon the epithelial surface or into the tubular lumen of the cauda region of adult rats. Furthermore, a positive staining for AChE in epithelial cells was also detected in the caput and cauda epididymis in all ages studied. Biochemical analysis confirmed a significant decrease in noradrenaline concentration as well as AChE activity in the cauda epididymis with sexual maturation. Immunohistochemical studies against microtubule-associated protein 1B (MAP 113), a neuronal cytoskeletal marker, further substantiated the quantitative changes observed in catecholaminergic and AChE-positive neuronal elements in the cauda epididymis. Thus, our results documented segment-specific variations in noradrenaline concentration and AChE activity during epididymal sexual maturation and suggest that such variations result, at least in part, from the refinement of the autonomic innervation pattern with age.
ISSN: 0196-3635
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Appears in Collections:Artigo

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