Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/44020
Title: High serum levels of soluble Fas (sFas) in CKD patients: Effects of renal clearance, reabsorption and synthesis
Authors: Dalboni, Maria Aparecida [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Manfredi, Silvia Regina [UNIFESP]
Andreoli, Maria Claudia Cruz [UNIFESP]
Santos, Oscar Fernando Pavão dos [UNIFESP]
Canziani, Maria Eugênia Fernandes [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
Góes, Miguel Ângelo [UNIFESP]
Draibe, Sergio Antonio [UNIFESP]
Balakrishnan, V.
Cendoroglo Neto, Miguel [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Tufts Univ New England Med Ctr
Keywords: sFas
RBP
sFasmRNA
chronic kidney diseases
Issue Date: 1-May-2008
Publisher: Wichtig Editore
Citation: International Journal Of Artificial Organs. Milan: Wichtig Editore, v. 31, n. 5, p. 405-410, 2008.
Abstract: Purpose: Increased serum concentrations of soluble Fas (sFas) have been reported in patients with chronic kidney disease (CKD). However, little is known about the renal clearance of sFas, whether sFas is reabsorbed in the renal tubules, or the behavior of sFas synthesis in CKD.Materials and methods: We studied 69 patients with CKD (60 +/- 15 years old, creatinine clearance 37+19 ml/min/1.73 m(2)) and 14 healthy subjects (61 +/- 17 years, creatinine clearance 79 +/- 24 ml/min/1.73 m(2)). ELISA was used to measure the levels of sFas (pg/mL) and retinol binding protein (RBP - mg/L). RT-PCR was used to quantify sFasmRNA of leukocytes.Results: Serum sFas levels were significantly higher in patients with CKD (2781 +/- 1214 vs. 2196 +/- 773, p=0.02). The concentrations of sFas in 24-hour urine samples (23 +/- 27 vs. 40 +/- 17, p=0.006) and sFas Clearance (0.019 +/- 0.022 vs. 0.036 +/- 0.020, p=0.01) were significantly lower in patients with CKD. sFas clearance correlated with creatinine clearance (r=0.25, p=0.02). Urine concentrations of RBP correlated with sFas concentrations in the urine (r=0.80, p<0.001). sFasmRNA were higher in patients with CKD (3.9 +/- 1.8 vs. 2.5 +/- 0.9, p<0.001).Conclusions: In CKD patients, the decrease in renal function is followed by a decrease in sFas clearance and an increase in serum sFas. In patients with proximal tubule dysfunction (high urinary RBP concentrations), urinary sFas is also increased, suggesting that sFas is reabsorbed by the proximal tubule. It is possible that an increase in sFas synthesis also contributes to the increase of serum sFas concentrations in uremia.
URI: http://repositorio.unifesp.br/11600/44020
ISSN: 0391-3988
Other Identifiers: http://www.artificial-organs.com/article/high-serum-levels-of-soluble-fas--sfas--in-ckd-patients--effects-of-renal-clearance--reabsorption-and-synthesis-art002304
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