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Title: Structure of heparan sulfate: Identification of variable and constant oligosaccharide domains in eight heparan sulfates of different origins
Authors: Dietrich, Carl Peter [UNIFESP]
Tersariol, Ivarne Luis dos Santos [UNIFESP]
Toma, Leny [UNIFESP]
Moraes, Carlos Torres [UNIFESP]
Porcionatto, Marimélia Aparecida [UNIFESP]
Oliveira, Fernanda Wanderley de [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: heparan sulfate, structure
heparan sulfate, disaccharide sequence
heparan sulfates, structural similarities
heparan sulfate, oligosaccharide domains
Issue Date: 1-May-1998
Publisher: Cellular & Molecular Biology
Citation: Cellular And Molecular Biology. Noisy-le-grand: Cellular & Molecular Biology, v. 44, n. 3, p. 417-429, 1998.
Abstract: The use of specific enzymes (heparinase and heparitinases from Flavobncterium heparinum, endoglucuronidase, alpha N-acetylglucosaminidase and beta-glucuronidase from the mollusc Anomalocardia brasiliana) and chemical methods (nitrous acid degradation, hydrazine N-deacetylation and borohydride reduction), led to the proposal of the total sequence of a heparan sulfate derived from bovine pancreas and partial sequences of heparan sulfates from different origins (bovine: lung, liver, brain; hog: liver, brain; rabbit liver; dog liver). It was shown that all the heparan sulfates contain common structural features such as: a N-acetylated and a N-sulfated domain made of glucuronic acid-containing disaccharides and a more sulfated region made of iduronic acid-containing disaccharides. Separating the two domains a peculiar tetrasaccharide made of GlcNAc-(alpha 1-4)-IdoUA-(alpha 1-4)-GlcNS-(alpha 1-4)-IdoUA was identified in all the heparan sulfates analyzed. It was also shown that the non-reducing ends of the heparan sulfates contain the monosaccharides glucosamine N-sulfate and/or glucosamine 2,6 disulfate.
ISSN: 0145-5680
Appears in Collections:Artigo

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