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Title: Galectin-3 as an immunohistochemical tool to distinguish pilocytic astrocytomas from diffuse astrocytomas, and glioblastomas from anaplastic oligodendrogliomas
Authors: Neder, Luciano
Marie, Suely Kazue Nagahashi [UNIFESP]
Carlotti, Carlos Gilberto
Gabbai, Alberto Alain [UNIFESP]
Rosemberg, Sergio
Malheiros, Suzana Maria Fleury [UNIFESP]
Siqueira, Rodrigo Proto
Oba-Shinjo, Sueli Mieko [UNIFESP]
Uno, Miyuki
Aguiar, Paulo Henrique [UNIFESP]
Miura, Flavio
Chammas, Roger [UNIFESP]
Colli, Benedicto Oscar
Silva Junior, Wilson Araujo
Zago, Marco Antonio
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Oct-2004
Publisher: Int Soc Neuropathology
Citation: Brain Pathology. Los Angeles: Int Soc Neuropathology, v. 14, n. 4, p. 399-405, 2004.
Abstract: The distinction of astrocytomas and oligodendrogliomas, mainly pilocytic astrocytomas (PILOs) from infiltrating astrocytomas and oligodendrogliomas (ODs), and high-grade oligodendrogliomas from glioblastomas (GBMs), poses a serious clinical problem. There is no useful immunohistochemical (IHC) marker to differentiate these gliomas, and sometimes the differential diagnosis between them is arbitrary. We identified galectin-3 (Gal-3) as a possible tool to differentiate them based on gene expression profiles of GBMs. We confirmed the differential expression in 45 surgical samples (thirteen GBMs; seven PILOs; 5 grade II ODs; 5 anaplastic oligodendrogliomas [AODs], including 2 Oligo-astrocytomas; 8 diffuse astrocytomas [ASTs], and 7 non-neoplastic samples) by quantification of Gal-3 gene expression by real-time quantitative PCR (rt-PCR). Higher expression of Gal-3 was observed in GBMs and PILOs than in OD, AODs and ASTs. The IHC expression of Gal-3 was evaluated in 90 specimens (fifteen PILOs, fourteen ASTs, 10 anaplastic astrocytomas, fifteen GBMs, eleven ODs, fifteen AODs, and 10 dysembryoplastic neuroepithelial tumors). The mean labeling score for Gal-3 determined according to the percentage of labeled cells in the tumor bulk was significantly different in GBMs versus AODs and in PILOs versus ASTs. Hence, Gal-3 is differentially expressed in central nervous system tumors, making IHC detection of Gal-3 a useful tool in distinguishing between these gliomas.
ISSN: 1015-6305
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