Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/42838
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dc.contributor.authorGastaldi, Ada Clarice [UNIFESP]
dc.contributor.authorJardim, José Roberto [UNIFESP]
dc.contributor.authorKing, M.
dc.date.accessioned2018-06-15T14:04:27Z-
dc.date.available2018-06-15T14:04:27Z-
dc.date.issued2000-01-01
dc.identifierhttp://content.iospress.com/articles/biorheology/bir066
dc.identifier.citationBiorheology. Amsterdam: Ios Press, v. 37, n. 3, p. 203-211, 2000.
dc.identifier.issn0006-355X
dc.identifier.urihttp://repositorio.unifesp.br/11600/42838-
dc.description.abstractFrozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a sample was thawed, studied and refrozen on each of the study days, at the same time that one sample was thawed only on the study day. Displacement in a simulated cough machine and on the frog palate, as well as contact angle measurements, were determined for the mucus samples on each study day. Mucus cytologic analyses on each of the study days were done with special regard to neutrophil counts and cell integrity. Friedman analysis of variance did not show any difference between the different periods of storage and the two temperatures for any of the parameters studied. The medians for the relative transport velocity on the frog palate varied between 0.88 and 1.03, for the contact angle between 21 and 28 degrees, and for the displacement in the simulated cough machine between 58 and 95 mm over the 90 days of the experiment. There were no cytologic alterations compatible with cell degeneration. We conclude that the storage of frog mucus either at -20 or -80 degrees C for periods up to 90 days does not lead to any significant differences in mucus transportability.en
dc.format.extent203-211
dc.language.isoeng
dc.publisherIos Press
dc.relation.ispartofBiorheology
dc.rightsAcesso restrito
dc.titleThe influence of temperature and length of time of storage of frog mucus samplesen
dc.typeArtigo
dc.rights.licensehttp://www.iospress.nl/service/authors/author-copyright-agreement/
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Alberta
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Ctr Univ Triangulo, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Div Resp, Sao Paulo, Brazil
dc.description.affiliationUniv Alberta, Dept Med, Pulm Res Grp, Edmonton, AB T6G 2M7, Canada
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Ctr Univ Triangulo, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Div Resp, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000088168300002
Appears in Collections:Artigo

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