Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/42831
Title: Fluorescence study of conformational properties of melanotropins labeled with aminobenzoic acid
Authors: Ito, A. S.
Souza, E. S. de
Barbosa, S. D.
Nakaie, Clovis Ryuichi [UNIFESP]
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Aug-2001
Publisher: Biophysical Society
Citation: Biophysical Journal. Bethesda: Biophysical Society, v. 81, n. 2, p. 1180-1189, 2001.
Abstract: The native hormone a-melanocyte-stimulating hormone (alpha -MSH) and its more potent analog [Nle(4),D-Phe(7)]alpha -MSH (NDP-alpha MSH), labeled at the amino terminal with the fluorescent aminobenzoic acid (Abz) isomers, were examined by fluorescence methods. We observed energy transfer between the tryptophan(9) residue acting as donor and Abz as acceptor, the transfer being more pronounced to the ortho-form of the acceptor. Within the hypothesis that different peptide conformations coexist in equilibrium during the fluorescence decay, we supposed that the intensity decay was modulated by an acceptor-donor distance distribution function f(r). From the time-resolved fluorescence experimental data, we recovered the distance distribution between Abz and Trp(9), using the CONTIN program, within the framework of the Forster resonance energy transfer model. The methodology proved to be useful to provide quantitative information about conformational dynamics of melanotropins and its dependency on the solvent. In aqueous medium, a-MSH has a broad Abz-Trp(9) distance distribution, reflecting the structural flexibility of the peptide. Three different distance populations could be identified in the labeled analog NDP-alpha MSH in water, indicating distinct conformational states for the synthetic peptide, compared with the native hormone. Measurements in trifluoroethanol resulted in the recovery of two Abz-Trp(9) distance populations, both for the native and the analog hormones, reflecting the decrease, induced by the solvent, of the conformational states available to the peptides.
URI: http://repositorio.unifesp.br/11600/42831
ISSN: 0006-3495
Appears in Collections:Artigo

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