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Title: DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil
Authors: Lima, Eleonidas Moura
Leal, Mariana Ferreira [UNIFESP]
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodríguez [UNIFESP]
Assumpcao, Paulo Pimentel [UNIFESP]
Bello, Maria Jose
Rey, Juan Antonio
Lima, Francinaldo Ferreira de
Casartelli, Cacilda
Univ Fed Piaui
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Hosp Joao Barros Barreto
Inst Invest Biomed
Keywords: gastric cancer
Issue Date: 1-Dec-2008
Publisher: Inst Histol Embriol-conicet
Citation: Biocell. Mendoza: Inst Histol Embriol-conicet, v. 32, n. 3, p. 237-243, 2008.
Abstract: Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric, cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric cancer samples. MLH1 and PMS2 methylation were associated with neoplastic samples compared to nonneoplastic ones. PMS2:? methylation was associated with diffuse- and intestinal-type cancer compared with normal controls. Intestinal-type cancer showed significant association with MLH1 methylation. Diffuse-type cancer was significantly associated with MSH2 methylation. Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that methylation is associated with gastric carcinogenesis. Methylation of mismatch repair genes was associated with gastric carcinogenesis and may be a helpful tool for diagnosis, prognosis and therapies. However, MSH6 does not seem to be regulated by methylation in our samples.
ISSN: 0327-9545
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