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Title: Demonstration of epithelial-mesenchymal transition in kidney - The contribution of a coupled histochemical and immunohistochemical staining with Periodic Acid-Thionin Schiff
Authors: Santos, Maria Carmen Lopes Ferreira Silva
Musso, Carlos
Alves, Venâncio Avancini Ferreira [UNIFESP]
Zatz, Roberto [UNIFESP]
Fujihara, Clarice Kazue
Malheiros, Denise Maria Avancini Costa
Inst Patol
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Espirito Santo Sch Med
Universidade de São Paulo (USP)
Keywords: periodic acid-thionin Schiff
periodic acid Schiff reaction
tubular basement membrane disruption
double immunostaining
epithelial-mesenchymal transition
Issue Date: 1-Mar-2008
Publisher: Lippincott Williams & Wilkins
Citation: Applied Immunohistochemistry & Molecular Morphology. Philadelphia: Lippincott Williams & Wilkins, v. 16, n. 2, p. 191-195, 2008.
Abstract: Traditional Periodic Acid Schiff has been extensively used, coupled with immunohistochemistry for epithelia or mesenchymal cells, to highlight renal tubular basement membrane (TBM). We recently tried to perform such technique in a 5/6 nephrectomy model of progressive renal fibrosis to demonstrate TBM disruption as an evidence for epithelial-mesenchymal transdifferentiation. Despite excellent basement membrane staining with traditional fuchsin-Periodic Acid Schiff, the interface between epithelial and mesenchymal cells was frequently blurred when revealed with 3'3 diaminobenzidine tetrachloride-peroxidase. Also, it was inadequate when revealed with alkaline phosphatase-fast red. We devised a triple staining method with Periodic Acid-Thionin Schiff to highlight basement membrane in blue, after double immunostaining for epithelium and mesenchymal cells. Blue basement membrane rendered a brisk contrast and highlighted boundaries between epithelial-mesenchymal interfaces. This method was easy to perform and useful to demonstrate the TBM, yield a clear demonstration of the very focal TBM disruption found in this model of progressive renal fibrosis.
ISSN: 1062-3345
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Appears in Collections:Artigo

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