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|Title:||Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice|
|Authors:||Oshima, Celina Tizuko Fujiyama [UNIFESP]|
Ribeiro, Daniel Araki [UNIFESP]
Gomes, Thiago Simao [UNIFESP]
Adios, Priscila C. [UNIFESP]
Egami, Mizue Imoto [UNIFESP]
Segreto, Helena Regina Comodo [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
|Publisher:||Int Inst Anticancer Research|
|Citation:||Anticancer Research. Athens: Int Inst Anticancer Research, v. 35, n. 5, p. 2817-2822, 2015.|
|Abstract:||Background/Aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. Materials and Methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. Results. In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+) group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.|
|Appears in Collections:||Artigo|
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