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Title: Iohexol Clearance for Determination of Glomerular Filtration Rate in Rats Induced to Acute Renal Failure
Authors: Passos, Michelle T. [UNIFESP]
Nishida, Sonia Kiyomi [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
Shimizu, Maria Heloisa
Mastroianni Kirsztajn, Gianna [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Issue Date: 13-Apr-2015
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 10, n. 4, 8 p., 2015.
Abstract: IntroductionThe glomerular filtration rate (GFR) is considered an especially important tool for the measurement of renal function. Inulin clearance (InCl) is the classic reference method for this purpose, although it is associated with a number of disadvantages; thus, other markers have been proposed, including iohexol. Determination of iohexol clearance (IoCl) has been established for clinical use; however, its application as a GFR marker in experimental rat models has not been reported.ObjectivesThis study aims to standardize a methodology for the measurement of iohexol clearance and to evaluate its applicability as a marker of GFR in rats with induced toxic acute renal failure (ARF), using InCl as the gold standard.Materials and MethodsTwenty-six Wistar male rats (200-300 g) were divided into the following two groups: a control group (n=7) and an ARF group (n=19). ARF was induced by the subcutaneous administration of cisplatin (5 mg/kg); IoCl and InCl were determined simultaneously, and plasma creatinine (pCreat) dosage was measured colorimetrically.ResultsThe pCreat, InCl and IoCl levels were consistent with the expected values for the renal function ranges of the evaluated animals, and the IoCl and InCl levels were significantly correlated (r=0.792). An inverse moderate linear correlation between the IoCl and pCreat measurements (r=-0.587) and between the InCl and pCreat measurements (r=-0.722) were observed.ConclusionThese results confirm a correlation between IoCl and the gold standard of GFR, InCl measurement. IoCl offers a relevant advantage over InCl because determination of the former allows the animal to live after the procedure.
ISSN: 1932-6203
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