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Title: Easy Storage Strategies for Sporothrix spp. Strains
Authors: Nogueira Brilhante, Raimunda Samia
Silva, Natalya Fechine
Chaves de Lima, Rita Amanda
Caetano, Erica Pacheco
Alencar, Lucas Pereira de
Collares Maia Castelo-Branco, Debora de Souza
Bezerra Moreira, Jose Luciano
Bandeira, Silviane Praciano
Camargo, Zoilo Pires de [UNIFESP]
Rodrigues, Anderson Messias [UNIFESP]
Pinheiro Gomes Bandeira, Tereza de Jesus
Monteiro, Andre Jalles
Cordeiro, Rossana de Aguiar
Costa Sidrim, Jose Julio
Gadelha Rocha, Marcos Fabio
Univ Fed Ceara
Univ Estadual Ceara
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Apr-2015
Publisher: Mary Ann Liebert, Inc
Citation: Biopreservation and Biobanking. New Rochelle: Mary Ann Liebert, Inc, v. 13, n. 2, p. 131-134, 2015.
Abstract: The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis; 6 S. schenckii; 5 S. mexicana, and 3 S. globosa) in saline at 4 degrees C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 degrees C and -80 degrees C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 degrees C and at -20 degrees C did not alter the number of conidia, but lower conidium counts were observed at -80 degrees C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.
ISSN: 1947-5535
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Appears in Collections:Em verificação - Geral

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