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dc.contributor.authorSousa-Martins, Diogo [UNIFESP]
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.contributor.authorFigueiredo, Mafalda C.
dc.contributor.authorSa e Cunha, Claudia
dc.contributor.authorMota-Filipe, Helder
dc.contributor.authorLima Filho, Acácio Alves de Souza [UNIFESP]
dc.contributor.authorBelfort, Rubens Junior [UNIFESP]
dc.contributor.authorRodrigues, Eduardo [UNIFESP]
dc.contributor.authorMaia, Mauricio [UNIFESP]
dc.identifier.citationInvestigative Ophthalmology & Visual Science. Rockville: Assoc Research Vision Ophthalmology Inc, v. 56, n. 3, p. 1993-2000, 2015.
dc.description.abstractPURPOSE. To investigate and compare the mechanism by which lutein-based and synthetic intraocular dyes interact with their target membranes during ophthalmic surgeries.METHODS. Surrogate membrane models were used in order to simulate the different intraocular membranes: internal limiting membrane (ILM), vitreous, anterior capsule (AC), and epiretinal membrane (ERM). Different lutein-based dyes, such as Phacodyne, Retidyne, Retidyne Plus, and Vitreodyne were tested, as well as Trypan Blue (TB), Indocyanine Green (ICG), Brilliant Blue (BB), and Triamcinolone Acetonide (TA). the interactions between the film components occurring at the air-water interface were investigated with surface pressure-area isotherms and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS).RESULTS. With the exception of TA and ICG, none of the tested dyes revealed toxicity to the analyzed membranes. the interaction of TA with the vitreous model affected deeply the biointerface structure of the model. A significant condensation of the monolayer is noted when ICG contacted with ILM by the isotherms or even a solubilization of part of the monolayer toward the aqueous subphase. Retidyne Plus may provide the fluidization of the membrane, but maintains intact the structure of proteins present in the model.CONCLUSIONS. the present study demonstrates for the first time that lutein-based dyes interact through a physical mechanism of action with membrane models of structures present in human eye. On the other hand, the chemical interaction of synthetic dyes TA and ICG resulted in an alteration of the membrane models.en
dc.publisherAssoc Research Vision Ophthalmology Inc
dc.relation.ispartofInvestigative Ophthalmology & Visual Science
dc.rightsAcesso aberto
dc.subjectlutein-based dyesen
dc.subjectsynthetic dyesen
dc.subjectintraocular membranesen
dc.subjectLangmuir filmsen
dc.titleComparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyesen
dc.contributor.institutionUniv Lisbon
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv Lisbon, Coll Pharm, Dept Social Pharm, P-1649003 Lisbon, Portugal
dc.description.affiliationFed Univ São Paulo Unifesp, Escola Paulista Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo Unifesp, Dept Earth & Exacts Sci, São Paulo, Brazil
dc.description.affiliationUnifespFed Univ São Paulo Unifesp, Escola Paulista Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifespFed Univ São Paulo Unifesp, Dept Earth & Exacts Sci, São Paulo, Brazil
dc.description.sourceWeb of Science
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