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Title: Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus
Authors: Paranaiba, Larissa Ferreira
Assis, Rafael Ramiro de
Nogueira, Paula Monalisa
Torrecilhas, Ana Claudia [UNIFESP]
Campos, Joao Henrique [UNIFESP]
Silveira, Amanda Cardoso de Oliveira
Martins-Filho, Olindo Assis
Pessoa, Natalia Lima
Campos, Marco Antonio
Parreiras, Patricia Martins
Melo, Maria Norma
Gontijo, Nelder de Figueiredo
Soares, Rodrigo Pedro Pinto
Fiocruz MS
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Leishmania enriettii
Cavia porcellus
Innate immunity
Issue Date: 17-Jan-2015
Publisher: Biomed Central Ltd
Citation: Parasites & Vectors. London: Biomed Central Ltd, v. 8, 14 p., 2015.
Abstract: Background: Leishmania enriettii is a species non-infectious to man, whose reservoir is the guinea pig Cavia porcellus. Many aspects of the parasite-host interaction in this model are unknown, especially those involving parasite surface molecules. While lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) of Leishmania species from the Old and New World have already been described, glycoconjugates of L. enriettii and their importance are still unknown.Methods: Mice peritoneal macrophages from C57BL/6 and knock-out (TLR2 -/-, TLR4 -/-) were primed with IFN-gamma and stimulated with purified LPG and GIPLs from both species. Nitric oxide and cytokine production were performed. MAPKs (p38 and JNK) and NF-kB activation were evaluated in J774.1 macrophages and CHO cells, respectively.Results: LPGs were extracted, purified and analysed by western-blot, showing that LPG from L88 strain was longer than that of Cobaia strain. LPGs and GIPLs were depolymerised and their sugar content was determined. LPGs from both strains did not present side chains, having the common disaccharide Gal(beta 1,4) Man(alpha 1)-PO4. the GIPL from L88 strain presented galactose in its structure, suggestive of type II GIPL. On the other hand, the GIPL of Cobaia strain presented an abundance of glucose, a characteristic not previously observed. Mice peritoneal macrophages from C57BL/6 and knock-outs (TLR2 -/- and TLR4 -/-) were primed with IFN-gamma and stimulated with glycoconjugates and live parasites. No activation of NO or cytokines was observed with live parasites. On the other hand, LPGs and GIPLs were able to activate the production of NO, IL-6, IL-12 and TNF-alpha preferably via TRL2. However, in CHO cells, only GIPLs were able to activate TRL2 and TRL4. in vivo studies using male guinea pigs (Cavia porcellus) showed that only strain L88 was able to develop more severe ulcerated lesions especially in the presence of salivary gland extract (SGE).Conclusion: the two L. enriettii strains exhibited polymorphisms in their LPGs and GIPLs and those features may be related to a more pro-inflammatory profile in the L88 strain.
ISSN: 1756-3305
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