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Title: Homemade Capillary Electrophoresis Coupled to a Mass Spectrometer
Authors: Moraes, Janderson Aparecido de [UNIFESP]
Carrilho, Emanuel
Assunção, Nilson Antonio [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Keywords: mass spectrometry
capillary electrophoresis
Issue Date: 2-Jan-2015
Publisher: Taylor & Francis Inc
Citation: Journal of Liquid Chromatography & Related Technologies. Philadelphia: Taylor & Francis Inc, v. 38, n. 1, p. 36-43, 2015.
Abstract: A system was developed in our laboratory to couple capillary electrophoresis with mass spectrometry. the capillary electrophoresis system was equipped with a high voltage supply, and a microcontroller with assembly language programming was developed for the computational control of the system. the MS system was a commercial Thermo Finningan LCQ ion trap mass spectrometer. the robustness of the coupled system was evaluated using standard protein samples (lysozyme, aprotinin, and bovine albumin) and tryptic digests of lysozyme. the system showed positive results in terms of robustness, allowing for the separation of digested proteins and the identification of 33% of the total amino acids in a protein (6 of the 18 expected peptides). the limit of detection was in the order of 1 picomole (signal-to-noise ratio), which was considered satisfactory for this system. the system shows high versatility in tandem coupling and combinations with other analytical procedures.
ISSN: 1082-6076
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