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Title: Assessment of free fetal DNA concentration in maternal plasma during the first trimester of pregnancy: comparative study between EDTA and PPT tubes - pilot study
Authors: Chadud, Carolina Schneider
Araujo Junior, Edward [UNIFESP]
Martinhago, Ciro Dresh
Mello Andari, Viviane Cristina
Tedesco, Giselle Darahem
Silva Bussamra, Luiz Claudio [UNIFESP]
Aoki, Tsutomu
Med Coll Sci Santa Casa São Paulo FCMSCSP
Universidade Federal de São Paulo (UNIFESP)
RDO Med Diagnost
Keywords: EDTA
first trimester of pregnancy
free fetal DNA
Issue Date: 1-Jan-2015
Publisher: Informa Healthcare
Citation: Journal of Maternal-fetal & Neonatal Medicine. London: Informa Healthcare, v. 28, n. 2, p. 172-176, 2015.
Abstract: Objective: To compare ethylenediamine tetraacetic acid (EDTA) tubes and plasma preparation tubes (PPT) for evaluating maternal plasma during the first trimester of pregnancy.Methods: A cross-sectional study was conducted on 24 male fetuses in women between 6 and 14 weeks of pregnancy. Blood samples (10 mL) were collected and stored in EDTA and PPT tubes. Subsequently, the samples were centrifuged and sent for free fetal DNA extraction by means of the polymerase chain reaction (PCR) technique. the reactions were performed in a real time PCR machine for detecting the amplification products. the genome region chosen for performing the PCR reactions was a target specific for the Y chromosome, in which the DYS-14 marker was amplified only when the DNA was of male sex. the free fetal DNA concentration was given by the threshold cycle (TC). To compare the tubes, the paired Student t-test was used.Results: the mean gestational age was 11.08 +/- 2.30 weeks (range: 6-14). the mean TC for PPT was 30.08 +/- 1.05 (range: 27.08-32.61) and for EDTA, 30.23 +/- 0.96 (range: 28.01-32.09), but without statistical significance (p = 0.357).Conclusion: We did not observe any statistically significant difference in free fetal DNA concentration between the EDTA and PPT tubes.
ISSN: 1476-7058
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