Please use this identifier to cite or link to this item:
|Title:||Levels and complexity of IgA antibody against oral bacteria in samples of human colostrum|
|Authors:||Petrechen, L. N.|
Zago, F. H.
Sesso, M. L. T.
Bertoldo, B. B.
Silva, C. B.
Azevedo, K. P.
Pereira, S. A. de Lima
Geraldo-Martins, V. R.
Ferriani, V. P. L.
Nogueira, R. D.
Universidade Federal de São Paulo (UNIFESP)
|Citation:||Immunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 220, n. 1, p. 142-146, 2015.|
|Abstract:||Streptococcus mutans (SM) have three main virulence antigens: glucan binding protein B (gbpB), glucosyltransferase (Gtf) and antigens I/II (Ag I/II) envolved in the capacity of those bacteria to adhere and accumulate in the dental biofilm. Also, the glycosyltransferases 153 kDa of Streptococcus gordonii (SGO) and 170 kDa of Streptococcus sanguinis (SSA) were important antigens associated with the accumulation of those bacterias. Streptococcus mitis (SMI) present IgA1 protease of 202 kDa. We investigated the specificity and levels IgA against those antigens of virulence in samples of human colostrum. This study involved 77 samples of colostrum that were analyzed for levels of immunoglobulian A, M and G by Elisa. the specificity of IgA against extracts of SM and initials colonizators (SSA, SMI, SGO) were analyzed by the Western blot. the mean concentration of IgA was 2850.2 (+/- 2567.2) mg/100 mL followed by IgM and IgG (respectively 321.8 +/- 90.3 and 88.3 +/- 51.5), statistically different (p < 0.05). Results showed that the majority of samples had detectable levels of IgA antibodies to extracts of bacteria antigens and theirs virulence antigens. To SM, the GbpB was significantly lower detected than others antigens of SM (p < 0.05). High complexities of response to Ags were identified in the samples. There were no significant differences in the mean number of IgA-reactive Ags between the antigens (p > 0.4). So, the breast milk from first hours after birth presented significant levels of IgA specific against important virulence of antigens those oral streptococci, which can disrupt the installation and accumulation process of these microorganisms in the oral cavity. (C) 2014 Elsevier GmbH. All rights reserved.|
|Appears in Collections:||Em verificação - Geral|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.