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Title: Effects of low level laser therapy on attachment, proliferation, and gene expression of VEGF and VEGF receptor 2 of adipocyte-derived mesenchymal stem cells cultivated under nutritional deficiency
Authors: Oliveira, Tabata Santos de
Serra, Andrey Jorge
Manchini, Martha Trindade
Bassaneze, Vinicius
Krieger, Jose Eduardo
Camillo de Carvalho, Paulo de Tarso
Espíndola-Antunes, Daniela [UNIFESP]
Bocalini, Danilo Sales
Ferreira Tucci, Paulo José [UNIFESP]
Silva, Jose Antonio
Nove Julho Univ
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Sao Judas Tadeu
Keywords: Cell adhesion
Cell proliferation
Low-level laser therapy
Mesenchymal stem cell
Issue Date: 1-Jan-2015
Publisher: Springer
Citation: Lasers in Medical Science. London: Springer London Ltd, v. 30, n. 1, p. 217-223, 2015.
Abstract: Low-level laser therapy (LLLT) has been shown to increase the proliferation of several cell types. We evaluated the effects of LLLT on adhesion, proliferation, and gene expression of vascular endothelial growth factor (VEGF) and type 2 receptor of VEGF (VEGFR2) at mesenchymal stem cells (MSCs) from human (hMSCs) and rat (rMSCs) adipose tissues on nutritional deficiencies. A dose-response curve was performed with cells treated with laser Ga-Al-As (660 nm, 30 mW) at energy of 0.7 to 9 J. Cell adhesion and proliferation were quantified 20, 40, and 60 min after LLLT and 24, 72, and 120 h after cultivation. Gene expression was verified by RT-PCR after 2 h of LLLT. A minor nutritional support caused a significant decrease in proliferation and adhesion of hMSCs and rMSCs. However, at the lowest LLLT dose (0.7 J), we observed a higher proliferation in hMSCs at standard condition shortly after irradiation (24 h). Adhesion was higher in hMSCs cultivated in controlled conditions at higher LLLT doses (3 and 9 J), and rMSCs show a reduction in the adhesion on 1.5 to 9 J. On nutritional deprivation, a 9 J dose was shown to reduce proliferation with 24 h and adhesion to all culture times in rMSCs. VEGF and VEGFR2 were increased after LLLT in both cell types. However, hMSCs under nutritional deprivation showed higher expression of VEGF and its receptor after irradiation with other laser doses. in conclusion, LLLT on human and rat MSCs might upregulate VEGF messenger RNA (mRNA) expression and modulate cell adhesion and proliferation distinctively.
ISSN: 0268-8921
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