Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/38482
Title: Toxoplasma gondii Oral Infection Induces Intestinal Inflammation and Retinochoroiditis in Mice Genetically Selected for Immune Oral Tolerance Resistance
Authors: Furtado Dias, Raul Ramos
Queiroz de Carvalho, Eulogio Carlos
Silva Leite, Carla Cristina da
Tedesco, Roberto Carlos [UNIFESP]
Calabrese, Katia da Silva
Silva, Antonio Carlos
DaMatta, Renato Augusto
Sarro-Silva, Maria de Fatima
Univ Estadual Norte Fluminense Darcy Ribeiro UENF
Fiocruz MS
Univ Estadual N Fluminen
Universidade Federal de São Paulo (UNIFESP)
Universidade do Estado do Rio de Janeiro (UERJ)
Issue Date: 1-Dec-2014
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 9, n. 12, 24 p., 2014.
Abstract: Toxoplasmosis is a worldwide disease with most of the infections originating through the oral route and generates various pathological manifestations, ranging from meningoencephalitis to retinochoroiditis and inflammatory bowel disease. Animal models for these pathologies are scarce and have limitations. We evaluated the outcome of Toxoplasma gondii oral infection with 50 or 100 cysts of the ME-49 strain in two lines of mice with extreme phenotypes of susceptibility (TS) or resistance (TR) to immune oral tolerance. Therefore, the aim of this study was to evaluate the behaviour of TS and TR mice, orally infected by T. gondii, and determine its value as a model for inflammatory diseases study. Mortality during the acute stage of the infection for TR was 50% for both dosages, while 10 and 40% of the TS died after infection with these respective dosages. in the chronic stage, the remaining TS succumbed while TR survived for 90 days. the TS displayed higher parasite load with lower intestinal inflammation and cellular proliferation, notwithstanding myocarditis, pneumonitis and meningoencephalitis. TR presented massive necrosis of villi and crypt, comparable to inflammatory bowel disease, with infiltration of lymphoid cells in the lamina propria of the intestines. Also, TR mice infected with 100 cysts presented intense cellular infiltrate within the photoreceptor layer of the eyes, changes in disposition and morphology of the retina cell layers and retinochoroiditis. During the infection, high levels of IL-6 were detected in the serum of TS mice and TR mice presented high amounts of IFN-gamma and TNF-alpha. Both mice lineages developed different disease outcomes, but it is emphasized that TR and TS mice presented acute and chronic stages of the infection, demonstrating that the two lineages offer an attractive model for studying toxoplasmosis.
URI: http://repositorio.unifesp.br/handle/11600/38482
ISSN: 1932-6203
Other Identifiers: http://dx.doi.org/10.1371/journal.pone.0113374
Appears in Collections:Em verificação - Geral

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