Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/38301
Title: Exploring Potential Virulence Regulators in Paracoccidioides brasiliensis Isolates of Varying Virulence through Quantitative Proteomics
Authors: Castilho, Daniele G. [UNIFESP]
Chaves, Alison F. A. [UNIFESP]
Xander, Patricia [UNIFESP]
Zelanis, Andre [UNIFESP]
Kitano, Eduardo S.
Serrano, Solange M. T.
Tashima, Alexandre K. [UNIFESP]
Batista, Wagner L. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Keywords: Paracoccidioides brasiliensis
pathogenic fungus
proteomic analysis
virulence regulators
Issue Date: 1-Oct-2014
Publisher: Amer Chemical Soc
Citation: Journal of Proteome Research. Washington: Amer Chemical Soc, v. 13, n. 10, p. 4259-4271, 2014.
Abstract: Few virulence factors have been identified for Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis. in this study, we quantitatively evaluated the protein composition of P. brasiliensis in the yeast phase using minimal and rich media to obtain a better understanding of its virulence and to gain new insights into pathogen adaptation strategies. This analysis was performed on two isolates of the Pb18 strain showing distinct infection profiles in B10.A mice. Using liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis, we identified and quantified 316 proteins in minimal medium, 29 of which were overexpressed in virulent Pb18. in rich medium, 29 out of 295 proteins were overexpressed in the virulent fungus. Three proteins were found to be up-regulated in both media, suggesting the potential roles of these proteins in virulence regulation in P. brasiliensis. Moreover, genes up-regulated in virulent Pb18 showed an increase in its expression after the recovery of virulence of attenuated Pb18. Proteins up-regulated in both isolates were grouped according to their functional categories. Virulent Pb18 undergoes metabolic reorganization and increased expression of proteins involved in fermentative respiration. This approach allowed us to identify potential virulence regulators and provided a foundation for achieving a molecular understanding of how Paracoccidioides modulates the host-pathogen interaction to its advantage.
URI: http://repositorio.unifesp.br/handle/11600/38301
ISSN: 1535-3893
Other Identifiers: http://dx.doi.org/10.1021/pr5002274
Appears in Collections:Em verificação - Geral

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