Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/38285
Title: Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH
Authors: Santana, Fabiana Rodrigues de
Coelho, Cideli de Paula
Cardoso, Thayna Neves
Pérez, Elizabeth Cristina [UNIFESP]
Benites, Nilson Roberti
Laurenti, Marcia Dalastra
Bonamin, Leoni Villano
Univ Paulista
Univ Santo Amara
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Keywords: Leishmania (L.) amazonensis
Antimoniurn crudum
Homeopathy
Murine leishnnaniasis
Issue Date: 1-Oct-2014
Publisher: Elsevier B.V.
Citation: Homeopathy. Oxford: Elsevier B.V., v. 103, n. 4, p. 264-274, 2014.
Abstract: Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. the modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.Methods: We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 x 10(6) Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes present in the peritoneal washing fluid and spleen were analyzed by flow cytometry and histopathology, with histometry of the subcutaneous primary lesion, local lymph node and spleen. Immunohistochemistry was performed to quantify CD3 (T lymphocyte), CD45RA (B lymphocyte) and CD11b (phagocytes) positive cells.Results: in treated mice, during the acute phase, there was significant increase of the macroscopic lesion, associated to inflammatory edema, as well increase in the number of free amastigotes and B lymphocytes inside the lesion. Increase of B lymphocytes (predominantly B-2 cells) was also seen in the local lymph node, spleen and peritoneum. in the chronic phase, the inflammatory process in the infection focus was reduced, with reduced phagocyte migration and peritoneal increase of B-1a cells (precursors of B-2 immunoglobulin producers cells) and T CD8+ cells.Conclusion: the treatment of mice with Antimonium crudum 30cH induced a predominantly B cell pattern of immune response in Leishmania (L.) amazonensis experimental infection, alongside the increase of free amastigote forms number in the infection site. the clinical significance of this study is discussed, further studies are suggested.
URI: http://repositorio.unifesp.br/handle/11600/38285
ISSN: 1475-4916
Other Identifiers: http://dx.doi.org/10.1016/j.homp.2014.08.006
Appears in Collections:Em verificação - Geral

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