Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/38174
Title: Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins
Authors: Figueiredo, Carlos R. [UNIFESP]
Matsuo, Alisson L. [UNIFESP]
Massaoka, Mariana H. [UNIFESP]
Polonelli, Luciano
Travassos, Luiz R. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Parma
Recepta Biopharma
Keywords: Peptide
CDR
Cytotoxicity
Antitumor activity
Melanoma
Issue Date: 1-Sep-2014
Publisher: Elsevier B.V.
Citation: Peptides. New York: Elsevier B.V., v. 59, p. 14-19, 2014.
Abstract: Short synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitro and in vivo antitumor activity of synthetic peptides derived from conserved CDR sequences of different immunoglobulins against human tumor cell lines and murine B16F10-Nex2 melanoma aiming at the discovery of candidate molecules for cancer therapy. Four light-and heavy-chain CDR peptide sequences from different antibodies (C36-L1, HA9-H2, 1-H2 and Mg16-H2) showed cytotoxic activity against murine melanoma and a panel of human tumor cell lineages in vitro. Importantly, theyalso exerted anti-metastatic activity using a syngeneic melanoma model in mice. Other peptides (D07-H3, MN20v1, MS2-H3) were also protective against metastatic melanoma, without showing significant cytotoxicity against tumor cells in vitro. in this case, we suggest that these peptides may act as immune adjuvants in vivo. As observed, peptides induced nitric oxide production in bone-marrow macrophages showing that innate immune cells can also be modulated by these CDR peptides. the present screening supports the search in immunoglobulins of rather frequent CDR sequences that are endowed with specific antitumor properties and may be candidates to be developed as anti-cancer drugs. (C) 2014 Elsevier Inc. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/38174
ISSN: 0196-9781
Other Identifiers: http://dx.doi.org/10.1016/j.peptides.2014.06.007
Appears in Collections:Em verificação - Geral

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