Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/38015
Title: Multidrug-Resistant Nontuberculous Mycobacteria Isolated from Cystic Fibrosis Patients
Authors: Campanini Candido, Pedro Henrique
Nunes, Luciana de Souza
Marques, Elizabeth Andrade
Folescu, Tania Wrobel
Coelho, Fabrice Santana
Nogueira de Moura, Vinicius Calado
Silva, Marlei Gomes da
Gomes, Karen Machado
Silva Lourenco, Maria Cristina da
Aguiar, Fabio Silva
Chitolina, Fernanda
Armstrong, Derek T.
Leao, Sylvia Cardoso [UNIFESP]
Goncalves Neves, Felipe Piedade
Queiroz Mello, Fernanda Carvalho de
Duarte, Rafael Silva
Universidade Federal do Rio de Janeiro (UFRJ)
Univ Fed Rio Grande do Sul
Inst Fernandes Figueira Fiocruz
Universidade do Estado do Rio de Janeiro (UERJ)
Fundacao Oswaldo Cruz
Inst Doencas Torax
Johns Hopkins Univ
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal Fluminense (UFF)
Issue Date: 1-Aug-2014
Publisher: Amer Soc Microbiology
Citation: Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 52, n. 8, p. 2990-2997, 2014.
Abstract: Worldwide, nontuberculous mycobacteria (NTM) have become emergent pathogens of pulmonary infections in cystic fibrosis (CF) patients, with an estimated prevalence ranging from 5 to 20%. This work investigated the presence of NTM in sputum samples of 129 CF patients (2 to 18 years old) submitted to longitudinal clinical supervision at a regional reference center in Rio de Janeiro, Brazil. From June 2009 to March 2012, 36 NTM isolates recovered from 10 (7.75%) out of 129 children were obtained. Molecular identification of NTM was performed by using PCR restriction analysis targeting the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene, and susceptibility tests were performed that followed Clinical and Laboratory Standards Institute recommendations. for evaluating the genotypic diversity, pulsed-field gel electrophoresis (PFGE) and/or enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) was performed. the species identified were Mycobacterium abscessus subsp. bolletii (n = 24), M. abscessus subsp. abscessus (n = 6), Mycobacterium fortuitum (n = 3), Mycobacterium marseillense (n = 2), and Mycobacterium timonense (n = 1). Most of the isolates presented resistance to five or more of the antimicrobials tested. Typing profiles were mainly patient specific. the PFGE profiles indicated the presence of two clonal groups for M. abscessus subsp. abscessus and five clonal groups for M. abscesssus subsp. bolletii, with just one clone detected in two patients. Given the observed multidrug resistance patterns and the possibility of transmission between patients, we suggest the implementation of continuous and routine investigation of NTM infection or colonization in CF patients, including countries with a high burden of tuberculosis disease.
URI: http://repositorio.unifesp.br/handle/11600/38015
ISSN: 0095-1137
Other Identifiers: http://dx.doi.org/10.1128/JCM.00549-14
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