Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/37945
Title: Trypanosoma cruzi: Genome characterization of phosphatidylinositol kinase gene family (PIK and PIK-related) and identification of a novel PIK gene
Authors: Oliveira, Priscila [UNIFESP]
Lima, Fabio Mitsuo [UNIFESP]
Cruz, Mario Costa [UNIFESP]
Ferreira, Renata Carmona [UNIFESP]
Sanchez-Flores, Alejandro
Cordero, Esteban Mauricio [UNIFESP]
Cortez, Danielle Rodrigues [UNIFESP]
Ferreira, Eden Ramalho [UNIFESP]
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
Bahia, Diana [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Nacl Autonoma Mexico
Universidade Federal de Minas Gerais (UFMG)
Keywords: Phosphatidylinositol kinases
Trypanosoma cruzi
Target of rapamycin
FYVE domain
FYVE-PIK
PIK
Issue Date: 1-Jul-2014
Publisher: Elsevier B.V.
Citation: Infection Genetics and Evolution. Amsterdam: Elsevier B.V., v. 25, p. 157-165, 2014.
Abstract: Chagas disease is caused by the protozoan Trypanosoma cruzi which affects 10 million people worldwide. Very few kinases have been characterized in this parasite, including the phosphatidylinositol kinases (PIKs) that are at the heart of one of the major pathways of intracellular signal transduction. Recently, we have classified the PIK family in T. cruzi using five different models based on the presence of PIK conserved domains. in this study, we have mapped PIK genes to the chromosomes of two different T. cruzi lineages (G and CL Brener) and determined the cellular localization of two PIK members. the kinases have crucial roles in metabolism and are assumed to be conserved throughout evolution. for this reason, they should display a conserved localization within the same eukaryotic species. in spite of this, there is an extensive polymorphism regarding PIK localization at both genomic and cellular levels, among different T. cruzi isolates and between T. cruzi and Trypanosoma brucei, respectively. We showed in this study that the cellular localization of two PIK-related proteins (TOR1 and 2) in the T. cruzi lineage is distinct from that previously observed in T. brucei. in addition, we identified a new PIK gene with peculiar feature, that is, it codes for a FYVE domain at N-terminal position. FYVE-PIK genes are phylogenetically distant from the groups containing exclusively the FYVE or PIK domain. the FYVE-PIK architecture is only present in trypanosomatids and in virus such as Acanthamoeba mimivirus, suggesting a horizontal acquisition. Our Bayesian phylogenetic inference supports this hypothesis. the exact functions of this FYVE-PIK gene are unknown, but the presence of FYVE domain suggests a role in membranous compartments, such as endosome. Taken together, the data presented here strengthen the possibility that trypanosomatids are characterized by extensive genomic plasticity that may be considered in designing drugs and vaccines for prevention of Chagas disease. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/37945
ISSN: 1567-1348
Other Identifiers: http://dx.doi.org/10.1016/j.meegid.2014.03.022
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