Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/37903
Title: Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Authors: Gegembauer, Gregory [UNIFESP]
Araujo, Leticia Mendes [UNIFESP]
Pereira, Edy Firmina
Rodrigues, Anderson Messias [UNIFESP]
Paniago, Anamaria Mello Miranda
Hahn, Rosane Christine
Camargo, Zoilo Pires de [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Univ Fed Mato Grosso UFMT
Issue Date: 1-Jul-2014
Publisher: Public Library Science
Citation: Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.
Abstract: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
URI: http://repositorio.unifesp.br/handle/11600/37903
ISSN: 1935-2735
Other Identifiers: http://dx.doi.org/10.1371/journal.pntd.0002986
Appears in Collections:Artigo

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