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Title: Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing
Authors: Monteiro, Jussimara [UNIFESP]
Widen, Raymond H.
Pignatari, Antonio C. C. [UNIFESP]
Kubasek, Carly
Silbert, Suzane
Tampa Gen Hosp
Universidade Federal de São Paulo (UNIFESP)
Keywords: Pyrosequencing
Real-time PCR
Klebsiella pneumoniae carbapenemase
Issue Date: 1-May-2014
Publisher: Elsevier B.V.
Citation: Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 100, p. 42-45, 2014.
Abstract: A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 b/alpha(Kpc) positive bacterial isolates were tested and characterized. in the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates. (c) 2014 Elsevier B.V. All rights reserved.
ISSN: 0167-7012
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Appears in Collections:Em verificação - Geral

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